Journal: Cancer Medicine

Article Title: Role of Ca 2+ ‐Dependent Epithelial‐Mesenchymal Transition in Malignant Progression of Colorectal Cancer: Special Focus on REG Iα/ EDNRB

doi: 10.1002/cam4.71754

Figure Lengend Snippet: REG Iα promotes cell migration, invasion, and EMT via EDNRB. (A) Cell migration was assessed by Transwell assay. (B) Matrigel‐coated Transwell assay assessed cell invasion. (C) Western blot analysis of EMT‐related markers, including the epithelial marker E‐Cadherin and mesenchymal markers N‐Cadherin and Vimentin. β‐actin was used as the loading control. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: CHOP (Abcam, ab317378), Cleaved‐Caspase 3 (Abcam, ab32042), Cleaved‐PARP (Cell Signaling Technology, 9541), E‐Cadherin (BOSTER, PB9561), N‐Cadherin (BOSTER, A01577‐3), and Vimentin (BOSTER, BM4029).

Techniques: Migration, Transwell Assay, Western Blot, Marker, Control

Journal: Cancer Medicine

Article Title: Role of Ca 2+ ‐Dependent Epithelial‐Mesenchymal Transition in Malignant Progression of Colorectal Cancer: Special Focus on REG Iα/ EDNRB

doi: 10.1002/cam4.71754

Figure Lengend Snippet: The REG Iα‐EDNRB axis promotes cell migration, invasion, and EMT via the Ca 2+ signaling pathway. (A) Transwell assay assessed cell migration. (B) Cell invasion was assessed by Matrigel‐coated Transwell assay. (C) EMT‐related protein levels (E‐Cadherin, N‐Cadherin, Vimentin) were detected by Western blot. β‐actin served as the loading control. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: CHOP (Abcam, ab317378), Cleaved‐Caspase 3 (Abcam, ab32042), Cleaved‐PARP (Cell Signaling Technology, 9541), E‐Cadherin (BOSTER, PB9561), N‐Cadherin (BOSTER, A01577‐3), and Vimentin (BOSTER, BM4029).

Techniques: Migration, Transwell Assay, Western Blot, Control

Journal: Cancer Medicine

Article Title: Role of Ca 2+ ‐Dependent Epithelial‐Mesenchymal Transition in Malignant Progression of Colorectal Cancer: Special Focus on REG Iα/ EDNRB

doi: 10.1002/cam4.71754

Figure Lengend Snippet: The REG Iα‐EDNRB‐Ca 2+ axis promotes tumor growth and EMT in vivo. (A) Representative images of the xenograft tumors and excised tumor tissues from the indicated groups. (B) Statistical analysis of the final tumor weights. (C) Tumor growth curves measuring tumor volume over time. (D) H&E staining showed pathological changes in tumor tissues. (E) TUNEL assay detected cell apoptosis in tumor tissues. (F) Cell proliferation in tumor tissues was shown by Ki67 IHC staining. (G) Expression of REG Iα (Immunofluorescence, upper row) and EDNRB (IHC, lower row) in tumor tissues. (H) Western blot analysis of REG Iα, EDNRB, and p‐CaMKII protein levels in tumor tissues. β‐actin was used as the loading control. (I) Western blot analysis of EMT‐related proteins (E‐Cadherin, N‐Cadherin, Vimentin) in tumor tissues. β‐actin was used as the loading control. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: CHOP (Abcam, ab317378), Cleaved‐Caspase 3 (Abcam, ab32042), Cleaved‐PARP (Cell Signaling Technology, 9541), E‐Cadherin (BOSTER, PB9561), N‐Cadherin (BOSTER, A01577‐3), and Vimentin (BOSTER, BM4029).

Techniques: In Vivo, Staining, TUNEL Assay, Immunohistochemistry, Expressing, Immunofluorescence, Western Blot, Control

Journal: The ocular surface

Article Title: Immuno Tomography (IT) and Imaging Mass Cytometry (IMC) for constructing spatially resolved, multiplexed 3D IMC data sets

doi: 10.1016/j.jtos.2022.04.008

Figure Lengend Snippet: Antibodies used for IMC.

Article Snippet: Gd158 , E-cadherin , Fluidigm , 24E10 , 3158029D , 1:100 , 5.

Techniques: Concentration Assay

Journal: International Journal of Biological Sciences

Article Title: Enhanced tumor immunotherapy by polyfunctional CD19-CAR T cells engineered to secrete anti-CD47 single-chain variable fragment

doi: 10.7150/ijbs.86632

Figure Lengend Snippet: Construction and characterization of CD19-CAR T and CD19-s47-CAR T cells. (A) Schematic representation of parental CD19-CAR and anti-CD47 secreting CD19-CAR (CD19-s47-CAR) constructs. CD19-CAR is successively connected by a CD8α signal peptide, an anti-CD19 scFv, a CD8α hinge, a CD8α transmembrane, a 4-1BB intracellular costimulatory domains and an intracellular CD3ζ domain. CD19-s47-CAR is composed of CD19-CAR linked with anti-CD47 scFv, and a HA tag was also included for detection of the secreted scFv. (B) Representative flow cytometry plots demonstrating CAR expression in human T cell. A fluorescently labeled CAR-specific antibodies (CARGREEN) was utilized to test the transfection efficiency. Untransduced T cells (UTDT) were used as a control. (C) Western blot was performed to analyze the expression of secreted anti-CD47 scFv in the supernatant from T cells. (D) Secreted antibodies in supernatants were enriched by HA immunoprecipitation kit and quantified by BCA quantification (n = 3). (E) Supernatants of CD19-s47-CAR T cells at different days post viral transfection were collected and quantified as in D and the concentration curve was plotted (n = 3). (F) Detection of the binding ability of secreted scFv in the culture medium (CM) to NHL cells by flow cytometry. NHL cells were incubated with culture medium from different T cells for 30 min at 37°C and then stained by anti-HA flow antibody. Representative flow charts are exhibited. (G) Visualization of anti-CD47 scFv binding to Raji cells by immunofluorescence. Scale bar, 50 μm. These experiments were performed at least three times with similar results.

Article Snippet: Culture supernatants of different T cells were collected and enriched for secreted anti-CD47 antibody by Anti-HA Tag Magnetic Beads Immunoprecipitation Kit (Sino Biological).

Techniques: Construct, Flow Cytometry, Expressing, Labeling, Transfection, Control, Western Blot, Immunoprecipitation, Concentration Assay, Binding Assay, Incubation, Staining, Immunofluorescence